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Tracking Operator State Fluctuations in Gene Expression in Single Cells

Banerjee, B and Balasubramanian, S and Ananthakrishna, G and Ramakrishnan, TV and Shivashankar, GV (2004) Tracking Operator State Fluctuations in Gene Expression in Single Cells. In: Biophysical Journal, 86 (5). pp. 3052-3059.

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We report the results of operator state fluctuations in gene expression for the entire bacterial growth cycle, using single-cell analysis and synthetic unregulated and negative-feedback transcription regulatory gene circuits. In the unregulated circuit, during the cell cycle, we observe a crossover from log-normal-to-normal distribution of expressed proteins and an unusual linear dependence of their standard deviation on the mean gene expression levels. With negative-feedback circuits we find the existence of bimodality as the cell cycle progresses. We suggest that such long-tail and bimodal distributions may be used as selection mechanisms in developmental switches and for assigning cell identity.

Item Type: Journal Article
Publication: Biophysical Journal
Publisher: The Biophysical Society.
Additional Information: Copyright of this article belongs to The Biophysical Society.
Department/Centre: Division of Chemical Sciences > Materials Research Centre
Division of Physical & Mathematical Sciences > Physics
Date Deposited: 19 Dec 2008 18:48
Last Modified: 19 Sep 2010 04:56
URI: http://eprints.iisc.ac.in/id/eprint/17358

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