Tracking Operator State Fluctuations in Gene Expression in Single Cells

Banerjee, B and Balasubramanian, S and Ananthakrishna, G and Ramakrishnan, TV and Shivashankar, GV (2004) Tracking Operator State Fluctuations in Gene Expression in Single Cells. In: Biophysical Journal, 86 (5). pp. 3052-3059.

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Abstract

We report the results of operator state fluctuations in gene expression for the entire bacterial growth cycle, using single-cell analysis and synthetic unregulated and negative-feedback transcription regulatory gene circuits. In the unregulated circuit, during the cell cycle, we observe a crossover from log-normal-to-normal distribution of expressed proteins and an unusual linear dependence of their standard deviation on the mean gene expression levels. With negative-feedback circuits we find the existence of bimodality as the cell cycle progresses. We suggest that such long-tail and bimodal distributions may be used as selection mechanisms in developmental switches and for assigning cell identity.

Item Type:	Journal Article
Publication:	Biophysical Journal
Publisher:	The Biophysical Society.
Additional Information:	Copyright of this article belongs to The Biophysical Society.
Department/Centre:	Division of Chemical Sciences > Materials Research Centre Division of Physical & Mathematical Sciences > Physics
Date Deposited:	19 Dec 2008 18:48
Last Modified:	19 Sep 2010 04:56
URI:	http://eprints.iisc.ac.in/id/eprint/17358

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