Rinderpest Virus RNA Polymerase Subunits: Mapping of Mutual Interacting Domains on the Large Protein L and Phosphoprotein P

Chattopadhyay, A and Shaila, MS (2004) Rinderpest Virus RNA Polymerase Subunits: Mapping of Mutual Interacting Domains on the Large Protein L and Phosphoprotein P. In: Virus Genes, 28 (2). pp. 169-178.

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Abstract

The RNA dependant RNA polymerase of negative sense RNA viruses is composed of two subunits - the Large protein ( L) and the Phosphoprotein ( P). These two proteins have to form a complex in order to carry out genome transcription and replication. Employing the baculovirus expression system, we demonstrate here, the specific in vivo interaction between the L and P proteins of Rinderpest virus and also the stabilization of L protein when it is present as L+P complex. The regions on either protein involved in such interaction has been studied using the yeast two-hybrid system which indicates that the P binding region resides within the amino terminal 380 amino acid residues of L protein. The L binding region on P protein has been mapped to lie within 347 - 490 amino acids.

Item Type:	Journal Article
Publication:	Virus Genes
Publisher:	Kluwer Academic Publishers.
Additional Information:	Copyright of this article belongs to Kluwer Academic Publishers.
Keywords:	Interaction;Large protein;Phosphoprotein;Rinderpest virus.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	19 Dec 2008 15:11
Last Modified:	19 Sep 2010 04:56
URI:	http://eprints.iisc.ac.in/id/eprint/17287

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