Decavanadate interacts with microsomal NADH oxidation system and enhances cytochrome c reduction

Ramasarma, T and Rao, Aparna V S (2006) Decavanadate interacts with microsomal NADH oxidation system and enhances cytochrome c reduction. In: Molecular And Cellular Biochemistry, 281 (1-2). pp. 139-144.

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Abstract

Oxidation of NADH with accompanying oxygen consumption (NADH:O-2 = 1:1) was observed in the combined presence of metavanadate (MV), decavanadate (DV) and microsomes.Oxygen consumption was negligible in the absence of MV, but NADH was oxidized and DV was reduced to a form of vanadyl-V-IV, colored blue like vanadyl sulfate but differed from it in having a 23-fold higher absorbance at 700 nm. DV can interact with the NADH oxidation system of microsomes as an electron acceptor,in addition to the known ferricyanide and cytochrome c. DV enhances rate of cytochrome c reduction significantly at mu M concentrations. These studies indicate potential of DV as a redox intermediate.

Item Type:	Journal Article
Publication:	Molecular And Cellular Biochemistry
Publisher:	Springer
Additional Information:	Copyright of this article belonggs to springer.
Keywords:	cytochrome c - decavanadate - NADH oxidation.
Department/Centre:	Division of Chemical Sciences > Solid State & Structural Chemistry Unit Division of Biological Sciences > Biochemistry
Date Deposited:	18 Mar 2009 11:58
Last Modified:	19 Sep 2010 04:55
URI:	http://eprints.iisc.ac.in/id/eprint/17054

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