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Rapid measurement of $^3J(H^N-H^\alpha)$ and $^3J(N-H^\beta)$ coupling constants in polypeptides

Barnwal, Ravi Pratap and Rout, Ashok K and Chary, KVR and Atreya, Hanudatta S (2007) Rapid measurement of $^3J(H^N-H^\alpha)$ and $^3J(N-H^\beta)$ coupling constants in polypeptides. In: Journal of Biomolecular NMR, 39 (4). pp. 259-263.

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We present two NMR experiments, (3,2)D HNHA and (3,2)D HNHB, for rapid and accurate measurement of $(^3J(H^N-H^\alpha)$ and $^3J(N-H^\beta)$ coupling constants in polypeptides based on the principle of G-matrix Fourier transform NMR spectroscopy and quantitative J-correlation. These experiments, which facilitate fast acquisition of three-dimensional data with high spectral/digital resolution and chemical shift dispersion, will provide renewed opportunities to utilize them for sequence specific resonance assignments, estimation/characterization of secondary structure with/without prior knowledge of resonance assignments, stereospecific assignment of prochiral groups and 3D structure determination, refinement and validation. Taken together, these experiments have a wide range of applications from structural genomics projects to studying structure and folding in polypeptides.

Item Type: Journal Article
Publication: Journal of Biomolecular NMR
Publisher: Springer
Additional Information: Copyright of this article belongs to Springer.
Keywords: Coupling constants;GFT NMR;Protein Structures;Protein folding;Resonance assignment
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Date Deposited: 18 Dec 2008 07:18
Last Modified: 28 Feb 2019 08:56
URI: http://eprints.iisc.ac.in/id/eprint/16526

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