YacG from Escherichia coli is a specific endogenous inhibitor of DNA gyrase

Sengupta, Sugopa and Nagaraja, Valakunja (2008) YacG from Escherichia coli is a specific endogenous inhibitor of DNA gyrase. In: Nucleic Acids Research, 36 (13). pp. 4310-4316.

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Abstract

We assign a function for a small protein, YacG encoded by Escherichia coli genome. The NMR structure of YacG shows the presence of an unusual zinc-finger motif. YacG was predicted to be a part of DNA gyrase interactome based on protein–protein interaction network. We demonstrate that YacG inhibits all the catalytic activities of DNA gyrase by preventing its DNA binding. Topoisomerase I and IV activities remain unaltered in the presence of YacG and its action appears to be restricted only to DNA gyrase. The inhibition of the enzyme activity is due to the binding of YacG to carboxyl terminal domain of GyrB. Overexpression of YacG results in growth inhibition and alteration in DNA topology due to uncontrolled inhibition of gyrase.

Item Type:	Journal Article
Publication:	Nucleic Acids Research
Publisher:	Oxford University Press
Additional Information:	Copyright of this article belongs to Sugopa Sengupta and Valakunja Nagaraja.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	18 Oct 2008 06:25
Last Modified:	19 Sep 2010 04:50
URI:	http://eprints.iisc.ac.in/id/eprint/16128

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