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Effect of different classes of inhibitors on DNA gyrase from Mycobacterium smegmatis

Chatterji, Monalisa and Unniraman, Shyam and Mahadevan, Sethuraman and Nagaraja, Valakunja (2001) Effect of different classes of inhibitors on DNA gyrase from Mycobacterium smegmatis. In: Journal of Antimicrobial Chemotherapy, 48 (4). pp. 479-485.

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Official URL: http://jac.oxfordjournals.org/cgi/reprint/48/4/479


Quinolones, coumarins, cyclothialidines, CcdB and microcin B17 inhibit DNA gyrase. Information regarding these various inhibitors comes from studies performed with the enzyme from Escherichia coli, and subsequent analyses have also primarily been confined to this system. We have carried out a detailed analysis of the effect of various groups of inhibitors on Mycobacterium smegmatis gyrase and emonstrate differential susceptibility of the E. coli and M. smegmatis gyrases. Interestingly, M. smegmatis gyrase was refractory to the plasmid-borne proteinaceous inhibitors CcdB and microcin B17. Ciprofloxacin, a fluoroquinolone, showed a 10-fold reduction in efficacy against M. smegmatis compared with E. coli gyrase. We have also shown that etoposide, an antineoplastic drug, inhibits DNA gyrase activity by trapping the gyrase-DNA complex. DNA gyrases from both E. coli and M. smegmatis were susceptible to etoposide at comparable levels.

Item Type: Journal Article
Publication: Journal of Antimicrobial Chemotherapy
Publisher: The British Society for Antimicrobial Chemotherapy
Additional Information: Copyright belongs to The British Society for Antimicrobial Chemotherapy.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 10 Oct 2008 14:06
Last Modified: 19 Sep 2010 04:50
URI: http://eprints.iisc.ac.in/id/eprint/16031

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