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Effect of Chain Length on Enzymatic Hydrolysis of \rho-Nitrophenyl Esters in Supercritical Carbon Dioxide

Varma, Mahesh N and Madras, Giridhar (2008) Effect of Chain Length on Enzymatic Hydrolysis of \rho-Nitrophenyl Esters in Supercritical Carbon Dioxide. In: Applied Biochemistry and Biotechnology, 144 (3). pp. 213-223.

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The effect of chain length on the enzymatic hydrolysis of various p-nitrophenyl esters was investigated. Specifically, the hydrolysis of various esters \rho-nitrophenyl butyrate (PNPB), \rho-nitrophenyl caprylate (PNPC), \rho-nitrophenyl laurate (PNPL), \rho-nitrophenyl myristate (PNPM) and \rho-nitrophenyl palmitate (PNPP) was studied in supercritical carbon dioxide $(ScCO_2)$ with lipase (Novozym 435). This indicates that the conversion of nitrophenyl esters decreases with increasing chain length. The effect of various parameters such as amount of water added, temperature, and enzyme loading was studied. The optimum temperature for the hydrolysis of PNPB and PNPC was 50°C but was 55°C for PNPL, PNPM, and PNPP in $ScCO_2$. The reactions were also conducted in acetonitrile as the solvent, and it was found that the reactions reach equilibrium much faster in $ScCO_2$ than in acetonitrile. The kinetics of the hydrolysis reactions were modeled using a Ping Pong Bi Bi model.

Item Type: Journal Article
Publication: Applied Biochemistry and Biotechnology
Publisher: Springer
Additional Information: Copyright of this article belongs to Springer.
Keywords: Hydrolysis;PNP;PNPL;Supercritical carbon dioxide;Equilibrium.
Department/Centre: Division of Mechanical Sciences > Chemical Engineering
Date Deposited: 18 Aug 2008
Last Modified: 27 Aug 2008 13:43
URI: http://eprints.iisc.ac.in/id/eprint/15633

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