Biological activity of single chain chorionic gonadotropin, HCG\alpha \beta, is decreased upon deletion of five carboxyl terminal amino acids of the \alpha subunit without affecting its receptor binding

Gupta, Sen C and Dighe, RR (2000) Biological activity of single chain chorionic gonadotropin, HCG\alpha \beta, is decreased upon deletion of five carboxyl terminal amino acids of the \alpha subunit without affecting its receptor binding. In: Journal of Molecular Endocrinology, 24 (2). pp. 157-164.

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Abstract

The strategy of translationally fusing the two subunits of human chorionic gonadotropin (hCG) has been used to produce recombinant single chain hCG in which the C-terminus of the alpha subunit is fused to the N-terminus \beta without any linker using Pichia pastoris expression system. The Pichia-expressed hCG\alpha\beta (phCG\alpha\beta) attained an overall conformation similar to that of hCG, and could bind to the receptor and elicit biological response, suggesting that receptor binding and signal transduction can take place even with a molecule having blocked the C-terminus of the \alpha subunit. The carboxyl terminal of the \alpha subunit has been shown to be involved in hormone binding and signal transduction of all the heterodimeric glycoprotein hormones. However, deletion of five amino acids from the C-terminus of the alpha subunit in the single chain hCG did not alter the overall conformation of the fusion molecule and its receptor binding ability, but led to a significant reduction in its ability to elicit biological response. These data show that these five amino acids at the C-terminus of the alpha subunit in the single chain hCG are not absolutely essential for attaining a conformation required for receptor binding, but are essential for obtaining a full biological response.

Item Type:	Journal Article
Publication:	Journal of Molecular Endocrinology
Publisher:	Society for Endocrinology
Additional Information:	Copyright of this article belongs to Society for Endocrinology.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	25 Aug 2008
Last Modified:	19 Sep 2010 04:49
URI:	http://eprints.iisc.ac.in/id/eprint/15623

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