Identification and characterization of a type II restriction endonuclease, Stri from streptomyces thermodiastaticus

Chandrashekaran, Siddamadappa and Shankar, Aparna Bhavani and Babu, Padmanabhan and Paul, Bindu Diana and Nagaraja, Valakunja (1999) Identification and characterization of a type II restriction endonuclease, Stri from streptomyces thermodiastaticus. In: Current Science, 77 (2). pp. 273-276.

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Abstract

A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5-C down arrow TCGAG-3 as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

Item Type:	Journal Article
Publication:	Current Science
Publisher:	Indian Academy of Sciences
Additional Information:	The copyright belongs to Indian Academy of Sciences.
Keywords:	endonuclease;streptomyces thermodiastaticus;enzyme
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	07 Mar 2005
Last Modified:	19 Sep 2010 04:15
URI:	http://eprints.iisc.ac.in/id/eprint/1541

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