Voltage-dependent gating of veratridine-modified RIIA Na+ channel \alpha subunit expressed heterologously in CHO cells

Ghatpande, AS and Sikdar, SK (1999) Voltage-dependent gating of veratridine-modified RIIA Na+ channel \alpha subunit expressed heterologously in CHO cells. In: Pflugers Archiv European Journal of Physiology, 438 (3). pp. 378-383.

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Abstract

The voltage-dependent kinetics of veratridine-modified RIIA Na+ channel alpha subunit expressed heterologously in CHO cells were studied using the whole-cell patch-clamp technique. The activation and deactivation kinetics are well described by double exponential functions but poorly by a monoexponential function. Unlike the slow component, the fast time constant and associated amplitude factor depended steeply on the potential. The steady-state activation of veratridine-modified channels is described by a Boltzmann function with a V-1/2 of -131.9 mV and a slope of 9.41 mV. A two-state model is proposed for the fast component that explains the kinetics of veratridines mechanism of action.

Item Type:	Journal Article
Publication:	Pflugers Archiv European Journal of Physiology
Publisher:	Springer Verlag
Additional Information:	Copyright for this article belongs to Springer Verlag.
Keywords:	kinetic analysis;patch clamp;RIIA sodium channel alpha subunit;veratridine induced modification
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	04 Mar 2005
Last Modified:	19 Sep 2010 04:15
URI:	http://eprints.iisc.ac.in/id/eprint/1538

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