Isolation and characterization of PR1 homolog from the genomic DNA of sandalwood (Santalum album L.)

Bhattacharya, Anirban and Sita, Lakshmi G (1999) Isolation and characterization of PR1 homolog from the genomic DNA of sandalwood (Santalum album L.). In: Current Science, 77 (7). pp. 959-963.

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Abstract

Genomic library was constructed using nuclear DNA prepared from tender leaves of sandalwood. Subsequently, screening with heterologous probes we. could isolate the PR1 genomic hemolog, Restriction mapping and hybridization experiments were carried out to obtain the coding region for PR1 gene. A 750 bp EcoRI fragment thus obtained was subcloned to yield pSaPR1, which was compared with the related sequences. Southern hybridization with genomic DNA digests was carried out to check its genomic organization. The induction of this gene was observed in the somatic embryos treated with salicylic acid, thereby implying its possible involvement during systemic acquired resistance.

Item Type:	Journal Article
Publication:	Current Science
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright for this article belongs to Indian Academy of Sciences.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	28 Jan 2005
Last Modified:	19 Sep 2010 04:15
URI:	http://eprints.iisc.ac.in/id/eprint/1514

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