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Temperature-dependent cell transformation in a calcium-binding protein from Entamoeba histolytica

Moorthy, Anu K and Gopal, B and Mohan, Gopi C and Murthy, MRN (1999) Temperature-dependent cell transformation in a calcium-binding protein from Entamoeba histolytica. In: Current Science, 77 (7). pp. 855-856.


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Temperature is a principle state variable which governs the structural, physical and functional properties of proteins to a large extent. We have observed an unusual temperature-dependent structural transition while attempting to collect X-ray diffraction data at room temperature (20°C) and at 4°C. This temperaturedependent cell transformation was observed in a calcium-binding protein (CaBP) from Entamoeba histolytica1,2. This CaBP is very similar to the structurally well-studdomains separated by a central linker. It is, however, smaller than CAM (134 residues) and has a two-residue insertion in the linker region. As a control, similar studies have also been carried out on small proteins like ribonuclease and lysozyme. These studies suggest that this kind of cell transformation is not a general phenomenon.

Item Type: Journal Article
Publication: Current Science
Publisher: Indian Academy of Sciences
Additional Information: The copyright belongs to Indian Academy of sciences.
Keywords: temperature;protein;entamoeba histolytica;x ray diffraction
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 19 Jan 2005
Last Modified: 19 Sep 2010 04:15
URI: http://eprints.iisc.ac.in/id/eprint/1513

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