Identification of functional domains of phosphoproteins of two morbilliviruses using chimeric proteins

Saikia, Paramananda and Shaila, MS (2008) Identification of functional domains of phosphoproteins of two morbilliviruses using chimeric proteins. In: Virus Genes, 37 (1). pp. 1-8.

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Abstract

The paramyxovirus P protein is an essential component of the transcriptase and replicase complex along with L protein. In this article, we have examined the functional roles of different domains of P proteins of two closely related morbilliviruses, Rinderpest virus (RPV) and Peste des petits ruminants virus (PPRV). The PPRV P protein physically interacts with RPV L as well as RPV N protein when expressed in transfected cells, as shown by co-immunoprecipitation.The heterologous L–P complex is biologically active when tested in a RPV minigenome replication/transcription system, only when used with PPRV N protein but not with RPV N protein. Employing chimeric PPRV/RPV cDNAs having different coding regions of P protein in the minigenome replication/transcription system, we identified a region between 290 and 346 aa in RPV P protein necessary for transcription of the minigenome.

Item Type:	Journal Article
Publication:	Virus Genes
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Rinderpest virus;Peste des petits ruminant virus;P protein;Transcription;Replication.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	18 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/15060

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