A novel association of a polymorphism in the first intron of adiponectin gene with type 2 diabetes, obesity and hypoadiponectinemia in Asian Indians

Vimaleswaran, Karani S and Radha, Venkatesan and Ramya, Kandaswamy and Babu, HNS and Savitha, Nageshappa and Roopa, Venkataramaiah and Monalisa, Dhar and Deepa, Raj and Ghosh, Saurabh and Majumder, Partha P and Rao, Sathyanarayana MR and Mohan, Viswanathan (2008) A novel association of a polymorphism in the first intron of adiponectin gene with type 2 diabetes, obesity and hypoadiponectinemia in Asian Indians. In: Human Genetics, 123 (6). pp. 599-605.

PDF fulltext.pdf Restricted to Registered users only Download (327kB) | Request a copy

Abstract

Adiponectin is an adipose tissue specifc protein that is decreased in subjects with obesity and type 2 diabetes. The objective of the present study was to examine whether variants in the regulatory regions of the adiponectin gene contribute to type 2 diabetes in Asian Indians.The study comprised of 2,000 normal glucose tolerant (NGT)and 2,000 type 2 diabetic, unrelated subjects randomly selected from the Chennai Urban Rural Epidemiology Study (CURES), in southern India. Fasting serum adiponectin levels were measured by radioimmunoassay. We identiWed two proximal promoter SNPs (-11377C \rightarrow G and -11282T \rightarrow C), one intronic SNP (+10211T \rightarrow G)and one exonic SNP (+45T!G) by SSCP and direct sequencing in a pilot study (n = 500). The +10211T \rightarrow G SNP alone was genotyped using PCR-RFLP in 4,000 study subjects. Logistic regression analysis revealed that subjects with TG genotype of +10211T \rightarrow G had signiWcantly higher risk for diabetes compared to TT genotype [Odds ratio 1.28; 95% ConWdence Interval (CI) 1.07–1.54; P = 0.008]. However, no association with diabetes was observed with GG genotype P = 0.22). Stratification of the study subjects based on BMI showed that the odds ratio for obesity for the TG genotype was 1.53 (95%CI 1.3–1.8; $P < 10^{-7}$) and that for GG genotype, 2.10 (95% CI 1.3–3.3; P = 0.002). Among NGT subjects, the mean serum adiponectin levels were signiWcantly lower among the GG (P = 0.007) and TG(P = 0.001) genotypes compared to TT genotype. Among Asian Indians there is an association of +10211T \rightarrow G polymorphism in the Wrst intron of the adiponectin gene with type 2 diabetes, obesity and hypoadiponectinemia.

Item Type:	Journal Article
Publication:	Human Genetics
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	21 Jul 2008
Last Modified:	25 Feb 2019 05:46
URI:	http://eprints.iisc.ac.in/id/eprint/14945

Actions (login required)

View Item