Changes in gene expression following androgen receptor blockade is not equivalent to androgen ablation by castration in the rat ventral prostate

Limaye, Anil M and Asangai, Irfan and Kalyani, Thyagarajan and Kondaiah, Paturu (2008) Changes in gene expression following androgen receptor blockade is not equivalent to androgen ablation by castration in the rat ventral prostate. In: Journal of Biosciences, 33 (2). pp. 209-220.

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Abstract

Involution of the rat ventral prostate and concomitant modulation of gene expression post-castration is a welldocumented phenomenon. While the rat castration model has been extensively used to study androgen regulation of gene expression in the ventral prostate, it is not clear whether all the gene expression changes post-castration are due to androgen depletion alone. To obtain insights into this, we performed differential display reverse transcriptase polymerase chain reaction (DD-RT-PCR) which resulted in the identifi cation of castration and/or flutamide-regulated genes in the rat ventral prostate. These include clusterin, methionine adenosyl transferase $II\alpha$, and prostate-specifi c transcripts such as PBPC1BS, S100RVP and A7. While clusterin, PBPC1BS and methionine adenosyl transferase $II\alpha$ are regulated by both castration and fl utamide, S100 RVP and A7 are regulated by castration alone. Interestingly, we show that fl utamide, unlike castration, does not induce apoptosis in the rat ventral prostate epithelium, which could be an underlying cause for the differential effects of castration and fl utamide treatment. We propose that castration leads to enrichment and depletion of stromal and epithelial cell types, respectively,resulting in erroneous conclusions on some of the cell type-specifi c transcripts as being androgen regulated.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	Castration;DD-RT-PCR;fl utamide;gene expression;rat ventral prostate.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	10 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/14928

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