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5-Fluorouracil modulates aminoacylation of rat liver tRNA

Vulmiri, Suryanarayana and Nayak, Rabindranath (1993) 5-Fluorouracil modulates aminoacylation of rat liver tRNA. In: Biochemistry & Molecular Biology International, 31 (6). pp. 1121-1128.

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Among the different classes of RNA, the effect of 5-fluorouracil (FUra), an anticancer drug, has been studied extensively on tRNA function in E. coli, but to a limited extent in eukaryotes, with specific reference to the mammalian system. Here the authors compared the aminoacylation function of rat liver tRNA substituted with FUra. Three hours after a single i.p injection of 50, 250 or 500 mg/kg body weight of FUra, total tRNAFUra150, tRNAFUra250, and tRNAFUra500, respectively, were isolated from the livers of 2-3 mo old male Wistar rats. The activity of tRNAFURa was compared with normal tRNA (tRNAN) isolated from saline-treated controls. TRNAFUra50 accepted [14C]-labeled total and five individual amino acids (lysine, aspartic acid, methionine, tryptophan, and serine) at a significantly higher rate compared to tRNAN. On the contrary, tRNAFUra250 and tRNAFUra500 displayed a dose-dependent inhibition in aminoacylation with total amino acids, lysine, and methionine. Acceptance of leucine was inhibited by tRNAFUra in a dose-dependent way. Overall, the amino acid acceptance was variable among the three populations of rat liver tRNAFUra isolated with varying doses of FUra and the possible reasons for the altered function of tRNA are discussed.

Item Type: Journal Article
Publication: Biochemistry & Molecular Biology International
Publisher: Taylor & Francis
Additional Information: Copyright of this article belongs to Taylor & Francis.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 10 Jul 2008
Last Modified: 27 Aug 2008 13:33
URI: http://eprints.iisc.ac.in/id/eprint/14876

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