An 18 mer sequence in a rat 1.3 kbp EcoRI repeat detects genetic polymorphism in humans

Ramachandra, Lakshmi and Devi, Radha Rama A and Rao, MRS (1993) An 18 mer sequence in a rat 1.3 kbp EcoRI repeat detects genetic polymorphism in humans. In: Current Science, 65 (7). pp. 569-571.

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Abstract

DNA fingerprinting involves the typing of an individual's DNA content to produce somatically stable,individual-specific DNA fingerprints. This technique often uses hypervariable minisatellite (HVMS) sequences as the fingerprinting probe and has found extensive use in several disciplines. Recently, we sequenced a 1.3 kbp EcoRI repetitive DNA fragment, shown to harbour the meiotic DNA repair site(s) of rat pachytene spermatocytes. This 1.3 kbp clone contained four sequences sharing high homology to the various HVMS sequences reported in the literature. Here we show th'at one of the sequences can indeed detect polymorphism in human individuals and can be used for DNA fingerprinting.

Item Type:	Journal Article
Publication:	Current Science
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	10 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/14873

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