Testis-specific Histone H1t Is Antigenically Distinct among H1 Subtypes

Markose, Elizabeth R and Rao, Manchanahalli R Satyanarayana (1985) Testis-specific Histone H1t Is Antigenically Distinct among H1 Subtypes. In: The Journal of Biological Chemistry, 260 (30). pp. 16263-16268.

PDF 16263.pdf Restricted to Registered users only Download (4MB) | Request a copy

Abstract

Histone H1t has been purified from rat testes and antibodies were elicited in rabbits. Immunoblotting studies with anti-histone H1t-IgG have shown that it reacted specifically with histone H1t but not with other histone H1 subtypes, namely H1a, -b, -c, -d, -e and H1O. The anti-histone H1t-IgG also did not react with chicken erythrocyte histone H5. Immunoblotting studies have also revealed that the polyclonal anti-histone H1t-IgG reacted with (a) two polypeptide fragments, NBS-N and NBS-C, derived from N-bromosuccinimide cleavage of histone H1t, (b)two polypeptide fragments, CT-N and CT-C, derived from $\alpha$-chymotrypsinc leavage of histone H1t, and (c) GH1t, globular domain of histone H1t obtained after trypsin cleavage. The indirect immunofluorescence studies on nuclei isolated from adult ratet stes with anti-histone H1t-IgG showed that the fluorescence, particularly, of the pachytene nucleus was the brightest. On the other hand, antihistone H1t-IgG did not stain nuclei from either liver or nuclei isolated fromth e testes of 10-day-old rats.

Item Type:	Journal Article
Publication:	The Journal of Biological Chemistry
Publisher:	American Society for Biochemistry and Molecular Biology
Additional Information:	Copyright of this article belongs to American Society for Biochemistry and Molecular Biology.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	03 Jul 2008
Last Modified:	19 Sep 2010 04:46
URI:	http://eprints.iisc.ac.in/id/eprint/14756

Actions (login required)

View Item