Agrobacterium-mediated genetic transformation in Gerbera hybrida

Nagaraju, V and Srinivas, GSL and Sita, Lakshmi G (1998) Agrobacterium-mediated genetic transformation in Gerbera hybrida. In: Current Science, 74 (7). pp. 630-634.

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Abstract

Agrobacterium-mediated genetic transformation protocol was developed for the commercially important ornamental Gerbera hybrida. Petiole, leaf and shoot tip explants from 3 to 4 week old in vitro grown shoots of gerbera were co-cultured with agrobacterium tumefaciens strain LBA4404. The strain harbours a binary vector containing neomycin p hosphotransferase (nptII) and 8-glucuronidase (uidA) genes. Callus formation and shoot regeneration were obtained on MS medium containing BAP, kinetin (1.0 mg/l each) and NAA (0.5 mg/l). Selection on the regeneration medium supplemented with 20 mg/l kanamycin allowed production of transgenic plants from 0.44% to 17.0% of the explants. GUS activity was detected by histochemical staining. Transfer of uidA and nptII genes was analysed by PCR and Southern hybridization. Transformed shoots were multiplied on MS medium containing 0.25 mg/l BAP, rooted in half strength MS medium supplemented with 40 mg/l kanamycin and successfully transferred to soil. This protocol couId be used to introduce horticulturally important genes that govern pigment biosynthesis in flowers.

Item Type:	Journal Article
Publication:	Current Science
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright for this article belongs to Indian Academy of Sciences.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	07 Feb 2005
Last Modified:	19 Sep 2010 04:15
URI:	http://eprints.iisc.ac.in/id/eprint/1475

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