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Peptidase N encoded by Salmonella enterica serovar Typhimurium modulates systemic infection in mice

Patil, Veerupaxagouda and Kumar, Anujith and Kuruppath, Sanjana and Nandi, Dipankar (2007) Peptidase N encoded by Salmonella enterica serovar Typhimurium modulates systemic infection in mice. In: FEMS Immunology & Medical Microbiology, 51 (2). pp. 431-442.

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The cytosolic protein degradation pathway, involving ATP-dependent proteases and ATP-independent peptidases, is important for modulating several cellular responses. The involvement of pathogen-encoded ATP-dependent proteases is well established during infection. However, the roles of ATP-independent peptidases in this process are not well studied. The functional role of Peptidase N (PepN), an ATP-independent enzyme belonging to the M1 family, during systemic infection of mice by Salmonella enterica serovar Typhimurium (Salmonella typhimurium) was investigated. In a systemic model of infection, the number of CFU of S. typhimurium containing a targeted deletion in peptidase N $(\Delta pepN)$, compared with wild type, was significantly higher in the lymph node and spleen. In addition, S. typhimurium replicated in the thymus and greatly reduced the number of immature $CD4^+CD8^+$ thymocytes in a dose- and time-dependent manner. Strains lacking or overexpressing pepN were used to show that the reduction in the number of thymocytes, but not lymph node cells, depends on a critical number of CFU. These findings establish a role for PepN in reducing the in vivo CFU of S. typhimurium during systemic infection. The implications of these results, in the context of the roles of proteases and peptidases, during host–pathogen interactions are discussed.

Item Type: Journal Article
Publication: FEMS Immunology & Medical Microbiology
Publisher: John Wiley & Sons, Inc.
Additional Information: Copyright of this article belongs to John Wiley & Sons, Inc.
Keywords: cytosolic protein degradation;enzyme;infection;Salmonella typhimurium
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 02 Jul 2008
Last Modified: 27 Aug 2008 13:31
URI: http://eprints.iisc.ac.in/id/eprint/14737

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