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NADH-dependent polyvanadate reduction by microsomes

Patole, Milind S and Kurup, Ramakrishna CK and Ramasarma, T (1987) NADH-dependent polyvanadate reduction by microsomes. In: Molecular and Cellular Biochemistry, 75 (2). pp. 161-167.

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NADH-dependent redn. of polyvanadate was obsd. by using rat liver microsomes as the enzyme source. The reduced vanadate obtained was blue in color, with a broad absorption max. in the red region at .apprx.650 nm. Microsomes and phosphate anions were essential for polyvanadate redn. The rate and the extent of formation of the blue-colored compd. depended on the amt. of vanadate present. Cytochrome b5 was involved in this superoxide dismutase-insensitive reaction. The rate of disappearance of the blue-colored compd. was dependent on the concn. of NADH and was sensitive to SOD. Catalase and $Mn^{2+}$, which inhibit the O consumption accompanying NADH oxidn., increased both the rate and extent of the blue-colored compd. formed. Vanadate apparently acts an electron acceptor.

Item Type: Journal Article
Publication: Molecular and Cellular Biochemistry
Publisher: Springer
Additional Information: Copyright of this article belongs to Springer.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 30 Jun 2008
Last Modified: 19 Sep 2010 04:46
URI: http://eprints.iisc.ac.in/id/eprint/14496

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