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Establishment of immunological probes to study human gonadotropin-releasing hormone receptors

Karande, AA and Rajeshwari, K and Schol, DJ and Hilgers, JHM (1995) Establishment of immunological probes to study human gonadotropin-releasing hormone receptors. In: Molecular and Cellular Endocrinology, 114 (1-2). pp. 51-56.

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Abstract

Monoclonal antibodies were raised to a synthetic peptide corresponding to amino acids 1–29 of the human gonadotropin-releasing hormone (GnRH) receptor. One of the two antibodies was found to recognise GnRH receptors on human pituitary gonadotrophs as determined by immunohistochemistry and supported by Western blotting. The antibody also bound to T47D human breast carcinoma cell line as determined by flow cytometric analysis.

Item Type: Journal Article
Publication: Molecular and Cellular Endocrinology
Publisher: Elsevier B.V.
Additional Information: Copyright belongs to Elsevier B.V.
Keywords: GnRH receptor peptide antibody;Pituitary gondotroph;Breast/ovarian carcinoma cell line
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 13 Jun 2008
Last Modified: 19 Sep 2010 04:45
URI: http://eprints.iisc.ac.in/id/eprint/14317

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