Mechanism of coupling periodate-oxidized nucleosides to proteins

Periannan, Senapathy and Mir, Ahmed A and Jacob, Mathai T (1985) Mechanism of coupling periodate-oxidized nucleosides to proteins. In: FEBS Letters, 190 (2). pp. 337-341.

Preview

PDF ddd.pdf Download (413kB)

Abstract

This paper describes a mechanism of coupling periodate-oxidized nucleosides to proteins. Each of the dialdehyde groups of a periodate-oxidized nucleoside is shown to couple to lysine residues on different protein molecules through Schiff bases, thereby cross-linking different protein molecules, forming a polymer. This is in contrast to the previous model in which nuclqosides were suggested to couple to proteins through a morpholine structure. The cross-linked structure of the nucleoside-antigen, significantly different when compared to the native protein, may affect the specificity and the efficiency of antibody production.

Item Type:	Journal Article
Publication:	FEBS Letters
Publisher:	Elsevier Science
Additional Information:	Copyright belongs to Elsevier Science
Keywords:	Nucleoside-antigen Periodate oxidation Schiff base Protein cross-linking Polymerization;Antibody production
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	11 Jun 2008
Last Modified:	19 Sep 2010 04:45
URI:	http://eprints.iisc.ac.in/id/eprint/14281

Actions (login required)

View Item