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Interaction of lutein with phosphatidylcholine bilayers

Chaturvedi, VK and Kurup, Ramakrishna CK (1986) Interaction of lutein with phosphatidylcholine bilayers. In: Biochimica et Biophysica Acta, 860 (2). pp. 286-292.


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The interaction of lutein, an inhibitory uncoupler of mitochondrial oxidative phosphorylation,with phospholipid liposome bilayers was examd. employing a variety of phys. methods.Differential calorimetric scans revealed that incorporation of lutein into aq. dispersions of dipalmitoylphosphatidylcholine broadened the main transition endotherm and decreased the transition enthalpy as well as the size of the cooperative unit without change in the temp. of transition.Permeability of the bilayer to ascorbate was enhanced significantly by the presence of lutein.The trapped vol. of the vesicles was decreased.Incorporation of lutein broadened the NMR peaks of both the acyl side-chain methylene and terminal Me protons without any change in the linewidth of the choline head-group Me proton signal.This indicated the ability of the compd. to integrate deep into the hydrophobic regions of the phospholipid bilayer distal to the polar regions.These results support earlier biochem. studies which revealed that the deleterious action of lutein on mitochondrial oxidative phosphorylation could be due to its ability to interact with membrane components and perturb the structure.

Item Type: Journal Article
Publication: Biochimica et Biophysica Acta
Publisher: Elsevier Science B.V.
Additional Information: Copyright belongs to Elsevier Science B.V.
Keywords: Lutein;Phosphofipid membrane;phase transition;ESR;Differential scanning calorimetry;NMR
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 09 May 2008
Last Modified: 19 Sep 2010 04:44
URI: http://eprints.iisc.ac.in/id/eprint/13912

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