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Generation of hydrogen peroxide by brown adipose tissue mitochondria

Sekhar, Seshadri B and Kurup, Ramakrishna CK and Ramasarma, T (1987) Generation of hydrogen peroxide by brown adipose tissue mitochondria. In: Journal of Bioenergetics and Biomembranes, 19 (43). pp. 397-407.

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This is the first report on the generation of $H_2O_2$ by brown adipose tissue mitochondria. Flavin dehydrogenase-linked substrates like succinate, glycerol-1-phosphate, and fatty acyl CoA were good substrates for the reaction, while $NAD^+$-linked substrates were less effective. In cold-acclimated animals the activity showed a substantial increase (2.5-fold). The $K_m$ and $V_{max}$ of the reaction were considerably lower than those of the respective dehydrogenase. Metal ions, particularly $Cu^{2+}$ and $Fe^{2+}$ were potent inhibitors of the reaction. Nucleoside diphosphates, which were inhibitors by themselves, potentiated the inhibitory action of $Fe^{2+}$ ions. In most of the properties, the $H_2O_2$ generator of brown adipose tissue mitochondria resembled that of liver mitochondria.

Item Type: Journal Article
Publication: Journal of Bioenergetics and Biomembranes
Publisher: Springer
Additional Information: Copyright of this article belongs to Springer.
Keywords: Cold exposure;thermogenesis;BAT mitochondria;H2O2 generator;GDP inhibition
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 05 May 2008
Last Modified: 27 Aug 2008 13:21
URI: http://eprints.iisc.ac.in/id/eprint/13828

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