SPR and ITC determination of the kinetics and the thermodynamics of bivalent versus monovalent sugar ligand–lectin interactions

Murthy, Bandaru Narasimha Murthy and Sinha, Sharmistha and Surolia, Avadhesha and Indi, Shantinath S and Jayaraman, Narayanaswamy (2007) SPR and ITC determination of the kinetics and the thermodynamics of bivalent versus monovalent sugar ligand–lectin interactions. In: Glycoconjugate Journal, 25 (4). pp. 313-321.

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Abstract

A kinetic study of the interaction of bivalent and monovalent sugar ligands with a lectin was undertaken with the aid of surface plasmon resonance (SPR) method. The study involved a series of bivalent α-d-mannopyranoside containing sugar ligands, with systematic variation in the distance between the sugar ligands. The detailed kinetic studies showed that bivalent ligands underwent a faster association $(k_{on})$ and a slower dissociation $(k_{off})$ of the ligand–lectin complexes, in comparison to the monovalent ligand–lectin complexes. The kinetic constants were complemented further by assessing the thermodynamic parameters with the aid of isothermal titration calorimetry (ITC). The initiation of cross-linking of ligand–lectin interactions emerge from the early stages of the complexation. The dynamic light scattering (DLS) and the transmission electron microscopy (TEM) techniques allowed judging the sizes and morphologies of the complex in the solution and solid states, respectively.

Item Type:	Journal Article
Publication:	Glycoconjugate Journal
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Lectins;Surface plasmon resonance;Isothermal titration calorimetry;Sugar ligands;Electron microscopy;Dynamic light scattering.
Department/Centre:	Division of Chemical Sciences > Organic Chemistry Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	25 Apr 2008
Last Modified:	19 Sep 2010 04:44
URI:	http://eprints.iisc.ac.in/id/eprint/13775

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