Plantlets from somatic callus tissue of the East Indian Rosewood (Dalbergia latif olia Roxb.)

Rao, Sankara K (1986) Plantlets from somatic callus tissue of the East Indian Rosewood (Dalbergia latif olia Roxb.). In: Plant Cell Reports, 5 (3). pp. 199-201.

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Abstract

Callus-mediated shoot bud formation was demonstrated in Dalbergia latifolia Roxb. (East Indian Rosewood). Cultures were raised from shoot explants of six year-old plants on Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA) and benzyladenine (BA). A sequential treatment of callus with increasing BA levels and decreasing NAA ensured shoot bud induction. Rooting of shoots was achieved by a three-step culture procedure involving 1) White's(W) liquid medium containing indoleacetic acid (IAA), naphthaleneacetic acid and indolebutyric acid (IBA), 2) half-strength MS agar-solidified medium with charcoal (0.25%) and 3) half-strength MS liquid medium.

Item Type:	Journal Article
Publication:	Plant Cell Reports
Publisher:	Springer Berlin / Heidelberg
Additional Information:	Copyright of this article belongs to Springer.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	24 Apr 2008
Last Modified:	19 Sep 2010 04:44
URI:	http://eprints.iisc.ac.in/id/eprint/13769

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