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Structural and thermodynamic consequences of introducing alpha-aminoisobutyric acid in the S peptide of ribonuclease S

Ratnaparkhi, Girish S and Awasthi, Satish Kumar and Rani, P and Balaram, P and Varadarajan, R (2000) Structural and thermodynamic consequences of introducing alpha-aminoisobutyric acid in the S peptide of ribonuclease S. In: Protein Engineering, 13 (10). pp. 697-702.

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The S protein–S peptide interaction is a model system to study binding thermodynamics in proteins. We substituted alanine at position 4 in S peptide by alpha-aminoisobutyric acid (Aib) to investigate the effect of this substitution on the conformation of free S peptide and on its binding to S protein. The thermodynamic consequences of this replacement were studied using isothermal titration calorimetry. The structures of the free and complexed peptides were studied using circular dichroic spectroscopy and X-ray crystallography, respectively. The alanine4Aib replacement stabilizes the free S peptide helix and does not perturb the tertiary structure of RNase S. Surprisingly, and in contrast to the wild-type S peptide, the G° of binding of peptide to S pro, over the temperature range 5–30°C, is virtually independent of temperature. At 25°C, the G°, H°, S and Cp of binding are 0.7 kcal/mol, 2.8 kcal/mol, 6 kcal/mol.K and –60 kcal/mol.K, respectively. The positive value of S is probably due to a decrease in the entropy of uncomplexed alanine4Aib relative to the wild-type peptide. The positive value of H° is unexpected and is probably due to favorable interactions formed in uncomplexed alanine4Aib. This study addresses the thermodynamic and structural consequences of a replacement of alanine by Aib both in the unfolded and complexed states in proteins.

Item Type: Journal Article
Publication: Protein Engineering
Publisher: Oxford University Press
Additional Information: Copyright for this article belongs to Oxford University Press
Keywords: Alpha-aminoisobutyric acid crystal structure;Conformational entropy;RNase S;Titration calorimetry
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 01 Sep 2004
Last Modified: 19 Sep 2010 04:14
URI: http://eprints.iisc.ac.in/id/eprint/1374

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