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Use of epoxysepharose for protein immobilisation

Murthy, GS and Moudgal, NR (1986) Use of epoxysepharose for protein immobilisation. In: Journal of Biosciences, 10 (3). pp. 351-358.


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Epoxy Sepharose, an activated affinity matrix which has been used for immobilisation of carbohydrates has been tried for immobilisation of proteins. Under normal conditions of coupling at neutral or alkaline pH proteins do not couple to epoxy Sepharose. However, a very high salt concentration during coupling allows the binding of proteins to epoxy Sepharose at a pH as low as 8·5. Increasing ionic strength and/or pH facilitates the binding. The bioactivity of the proteins is not destroyed by the immobilisation. This matrix, unlike cyanogen bromide-Sepharose, retains its ability to bind albumin by 80–90%even after 60 days of storage in aqueous suspension at $4^0C$. Its capacity to bind proteins is comparable to that of cyanogen bromide-Sepharose.

Item Type: Journal Article
Publication: Journal of Biosciences
Publisher: Indian Academy of Science
Additional Information: Copyright of this article belongs to Indian Academy of Science.
Keywords: Epoxy Sepharose;affinity matrix;protein immobilisation.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 13 Apr 2008
Last Modified: 19 Sep 2010 04:44
URI: http://eprints.iisc.ac.in/id/eprint/13724

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