Sivaramakrishnan, S and Panini, SR and Ramasarma, T (1983) Activation of succinate dehydrogenase in isolated mitochondria by noradrenaline. In: Indian Journal of Biochemistry & Biophysics, 20 (1). pp. 23-28.
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Activation of succinate dehydrogenase (EC 1.3.99.1) [9002-02-2] was obtained on preincubating rat liver mitochondria with noradrenaline (I) [51-41-2] and 3,4-dihydroxybenzoate [99-50-3], a compd. which could be derived from the amine. The KA for 3,4-dihydroxybenzoate (6.3 \times 10-4M) was one order of magnitude less than that of I (6.2 \times 10-3M). The activation was of the V type, with no change in the Km for succinate, the 1st of this type among the activators of succinate dehydrogenase. Addn. of I to the assay medium inhibited the enzyme activity but the kinetics of inhibition were different from those of the other known classes of activators of succinate dehydrogenase, including 3,4-dihydroxybenzoate. Experiments on washing activated mitochondria prepration indicated strong binding of both the activators, and the activation was reversed only after preincubation with succinate followed by 2 washings with the homogenizing medium. Mitochondria isolated from rat tissues, viz. heart, kidney, and brown adipose tissue, also showed activation with I. Liver mitochondria from cold-acclimated rats showed a sensitization effect giving the same max. activation at a lower concentration of I.
| Item Type: | Journal Article |
|---|---|
| Publication: | Indian Journal of Biochemistry & Biophysics |
| Publisher: | National Institute of Science Communication and Information Resources |
| Additional Information: | Copyright of this article belongs to National Institute of Science Communication and Information Resources |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 15 Apr 2008 |
| Last Modified: | 27 Aug 2008 13:19 |
| URI: | http://eprints.iisc.ac.in/id/eprint/13676 |
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