Reverse-Phase High-Performance Liquid Chromatographic Separation of Methylated and Non-Methylated Nucleic Acid Bases

Madyastha, Prema and Rao, Pratima and Deobagkar, DN and Madyastha, KM (1983) Reverse-Phase High-Performance Liquid Chromatographic Separation of Methylated and Non-Methylated Nucleic Acid Bases. In: Current Science, 52 (11). pp. 536-538.

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Abstract

A high-performance liquid chromatographic sepration method is described for the detection of 5-methylcytosine and 6-methyladenine in nucleic acid ext. The bases were sepd. on a Waters $C18 \mu$ Bondapak column with a water: methanol acetic acid system. Effluents were monitored by UV absorption at 254 nm. The bases were estd. by peak heights which are proportional to the amts. of the individual bases. The method is rapid, sensitive, easy to perform and reproducible.

Item Type:	Journal Article
Publication:	Current Science
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	10 Apr 2008
Last Modified:	19 Sep 2010 04:44
URI:	http://eprints.iisc.ac.in/id/eprint/13671

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