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Synthesis and Purification of Oligodeoxyribonucleotides: A Modified Approach

Majumder, Kumud and Kadalayil, Latha P and Brahmachari, SK (1987) Synthesis and Purification of Oligodeoxyribonucleotides: A Modified Approach. In: Current Science, 56 (14). pp. 693-697.

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A reduction in monomer consumption in the solid phase synthesis of oligodeoxyribonucleotides has been achieved by introducing a repeat coupling approach. Additionally, a method of anion exchange HPLC- purification of the synthesized nucleotides at room temperature has been developed in which a highly volatile buffer was used as the eluent. An alternative approach of HPLC purification of oligonucleotides is also reported.

Item Type: Journal Article
Publication: Current Science
Publisher: Indian Academy of Sciences
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 02 Apr 2008
Last Modified: 19 Sep 2010 04:44
URI: http://eprints.iisc.ac.in/id/eprint/13630

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