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DNA topoisomerase I from Mycobacterium smegmatis

Bhaduri, Tisha and Nagaraja, V (1994) DNA topoisomerase I from Mycobacterium smegmatis. In: Indian Journal of Biochemistry & Biophysics, 31 . pp. 339-343.


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DNA topoisomerase I has been purified from Mycobacterium smegmatis to near homogeneity using different column chromatographic techniques. The enzyme activity relaxes form I DNA into form IV DNA, requiring Mg +2, but not ATP or any other cofactors for its activity. Several properties of the enzyme were found to be similar to that of the prototype enzyme, Escherichia coli topoisomerase I.

Item Type: Journal Article
Publication: Indian Journal of Biochemistry & Biophysics
Publisher: CSIR, New Delhi
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 01 Sep 2004
Last Modified: 19 Sep 2010 04:14
URI: http://eprints.iisc.ac.in/id/eprint/1361

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