Transfection of Mycobacterium smegmatis SN2 with mycobacteriophage I3 DNA

Karnik, Sadashiva S and Gopinathan, KP (1983) Transfection of Mycobacterium smegmatis SN2 with mycobacteriophage I3 DNA. In: Archives of Microbiology, 136 (4). pp. 275-280.

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Abstract

Mycobacterium smegmatis SN2 does not exhibit natural competence for the uptake of phage I3 DNA. Competence can artificially be induced by treatment with glycine or $CaCl_2$, and the combination of both is even more effective. The efficiency of transfection can be improved by inclusion of protamine sulphate and heterologous RNA in the system. From $^{32}P$ DNA uptake studies the major barrier for the entry of DNA has been found to be the complex cell wall. The efficiency of transfection calculated on the basis of fraction of DNA which has entered the cell is comparable to that of other bacterial systems. The phage development takes a longer time (7 h for one cycle) after transfection, as compared to infection (4 h)

Item Type:	Journal Article
Publication:	Archives of Microbiology
Publisher:	Springer Berlin / Heidelberg
Additional Information:	copyrights of this article belongs to Springer.
Keywords:	Transfection; Mycobacterium smegmatis; Mycobacteriophage I3; Calcium sensitization; Glycine sensitization; Protamine sulfate; Exogenous RNA; DNA uptake.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	31 Mar 2008
Last Modified:	19 Sep 2010 04:44
URI:	http://eprints.iisc.ac.in/id/eprint/13551

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