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Hypermethylation and higher stability of a plant (Eleusine coracana) tRNA

Raviprakash, KS and Cherayil, Joseph D (1985) Hypermethylation and higher stability of a plant (Eleusine coracana) tRNA. In: International Journal of Biological Macromolecules, 7 (4). pp. 230-234.

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Total tRNA isolated from four-day-old ragi (Eleusin coracana) seedlings has been shown to be highly methylated. Each tRNA molecule on average contains two 2′-O-ribose methylated nucleosides. The high molar yields of 1-methyladenosine (1.6%) indicate that nearly a third of all the tRNA molecules contains more than one residue of 1-methyladenosine. Thermal denaturation studies with total tRNA show that the hypermethylated ragi tRNA melts slower that the yeast tRNA which is less methylated but otherwise has similar base composition. Ragi tRNA is also less susceptible to ribonucleases A, $T_2 and T_2$.

Item Type: Journal Article
Publication: International Journal of Biological Macromolecules
Publisher: Elsevier Science
Additional Information: Copyright belongs to Elsevier Science
Keywords: Ribonucleases;tRNA;modified nucleotides;Eleusin coracana
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 20 Mar 2008
Last Modified: 19 Sep 2010 04:43
URI: http://eprints.iisc.ac.in/id/eprint/13418

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