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Antibodies to guanylic acid: Fractionation and specificities

Iswari, S and Jacob, TM (1984) Antibodies to guanylic acid: Fractionation and specificities. In: Bioscience Reports, 4 (12). pp. 1027-1036.

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Guanylic-acid-specific antibodies were elicited in rabbits) using as immunogen pG linked through $5^{\prime}$-phosphate to thyroglobulin. Specificity and affinity of antibodies to nucleotides, nucleosides, DNA, and RNA were studied by their binding to radioactive ligands and competition experiments. Guanylic-acid-specific antibodies do not bind to deoxyguanylic acid and have an average association constant of $10^7 M^{-1}$ at 4°C. Binding of the antibodies to $^3H-RNA$ is G-specifiC. The antibodies do not bind to $^{32}P-ssDNA$ or $^{32}P-dsDNA$. The pG-specific antibodies could be separated into different fractions by affinity chromatography. These fractions) though specific to pG) differ in their cross-reactivities to nucleosides and nucleotides.

Item Type: Journal Article
Publication: Bioscience Reports
Publisher: Springer Netherlands
Additional Information: Copyright of this article belongs to Springer Netherlands.
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Division of Biological Sciences > Biochemistry
Date Deposited: 20 Mar 2008
Last Modified: 19 Sep 2010 04:43
URI: http://eprints.iisc.ac.in/id/eprint/13411

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