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Theoretical studies on the modes of binding of some of the derivatives of D-mannose to concanavalin A

Sekharudu, Chandra Y and Rao, VSR (1984) Theoretical studies on the modes of binding of some of the derivatives of D-mannose to concanavalin A. In: International Journal of Biological Macromolecules, 6 (6). pp. 337-347.

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The possible modes of binding for methyl-$\alpha$-$_D$-mannopyranoside, methyl-$\beta$-$_D$-mannopyranoside, 2-O-methyl-$\alpha$-$_D$mannopyranoside, methyl-2-O-methyl-$\alpha$-$_D$-mannopyranoside and methyl-$\alpha$-$_D$-N-acetylmannosamine to concanavalin A have been investigated using theoretical methods. All these sugars, except methyl-$\alpha$-$_D$-N-acetylmannosamine, reach the active site of concanavalin A with a highly restricted number of niding orientations. Present investigations suggest that the failure of methyl-$\alpha$-$_D$-N-acetylmammosamine to bind to concanavalin A is not so much due to steric factors as to repulsive electrostatic interactions. Methyl-2-O-methyl-$\alpha$-$_D$-mannopyranoside can bind to concanavalin A in one mode whereas the other sugars can bind in more than one mode. The high potency of methyl-$\alpha$-$_D$-mannopyranoside over methyl-$\beta$-$_D$-mannopyranoside is mainly due to the possibility of hydrophobic interactions of the α-methoxy group with Leu(99) or Tyr(100) and also due to the possibility of formation of better and more hydrogen bonds with the protein. A comparison of these data with those for the Image-glucopyranosides suggests that the change of the hydroxyl at the C-2 atom from equatorial to axial orientation increases the stereochemically allowed region as well as the possible binding modes. From these studies it is also suggested that the overall shape of the oligosaccharides rather than the terminal or internal mannose alone affects the binding potency of saccharides to concanavalin A.

Item Type: Journal Article
Publication: International Journal of Biological Macromolecules
Publisher: Elsevier Science B.V.
Additional Information: Copyright of this article belongs to Elsevier Science B.V.
Keywords: Lectin;concanavalin A;carbohydrate-lectin interaction;molecular fit
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 20 Mar 2008
Last Modified: 19 Sep 2010 04:43
URI: http://eprints.iisc.ac.in/id/eprint/13410

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