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Influence of formamide on the thermal stability of DNA

Sadhu, Chanchal and Dutta, Santanu and Gopinathan, KP (1984) Influence of formamide on the thermal stability of DNA. In: Journal of Biosciences, 6 (6). pp. 817-821.


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The utility of formamide in the denaturation and renaturation of DNA has been examined. The mmelting temperature of duplex DNA is lowered by 0.6°C per per cent of formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30% it does not affect the rate of DNA reassociation.

Item Type: Journal Article
Publication: Journal of Biosciences
Publisher: Indian Academy of Sciences
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Keywords: DNA melting;formamide;Tm;DNA base composition;DNA reassociation
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 20 Mar 2008
Last Modified: 19 Sep 2010 04:43
URI: http://eprints.iisc.ac.in/id/eprint/13409

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