Effect of vitamin A deprivation on the mitogenic factor activity in the rat testes

Unni, Emmanual and Kssari, KV and Rao, MRS (1984) Effect of vitamin A deprivation on the mitogenic factor activity in the rat testes. In: Biochemical and Biophysical Research Communications, 125 (2). pp. 454-462.

PDF effect_of_vit_A.pdf Restricted to Registered users only Download (492kB) | Request a copy

Abstract

The seminiferous tubules of rat testes contain a protease and heat sensitive factor capable of stimulating $(^3H)$-thymidine incorporation into quiescent cultures of NIH 3T3 mouse fibroblast cells. This mitogenic factor activity was only 4% in the testes of vitamin A deficient-retinoic acid maintained rats as compared to that of normal rats. Supplementation of retinyl acetate to these vitamin A deficient rats for 4 days resulted in a 56% recovery in the mitogenic factor activity while by day 16, the recovery was 80 percent. Incorporation of $(^3H)$-thymidine into DNA of the seminiferous tubules of vitamin A deficient rats was only 46% of that of normal rats which was restored to normal levels by retinyl acetate supplementation for 24 days.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier Inc
Additional Information:	Copyright of this article belongs to Elsevier Inc.
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	19 Mar 2008
Last Modified:	19 Sep 2010 04:43
URI:	http://eprints.iisc.ac.in/id/eprint/13400

Actions (login required)

View Item