Presence of mycobacteriophage 13-1ike DNA sequences in the genome of its host Mycobacterium smegmatis

Sadhu, C and Dutta, S and Gopinathan, KP (1986) Presence of mycobacteriophage 13-1ike DNA sequences in the genome of its host Mycobacterium smegmatis. In: Archives of Microbiology, 146 (2). pp. 166-169.

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Abstract

The genomes of phage 13 and its host Mycobacterium smegmatis have been compared. From thermal melting studies the GC contents of DNA from mycobacteriophage 13 and its host M. smegmatis were found to be 66%. A new method, based only on the initial rates of reassociation, has been developed for calculating the DNA homology. Analysis of DNA reassociation kinetics suggested the presence of one equivalent of the phage 13 genome within the M. smegmatis genome. Southern analysis revealed the presence of almost all of the phage 13 specific sequences within the host genome.

Item Type:	Journal Article
Publication:	Archives of Microbiology
Publisher:	Springer Berlin / Heidelberg
Additional Information:	Copyright of this article belongs to Springer Berlin / Heidelberg.
Keywords:	Mycobacterium smegmatis;Mycobacteriophage 13;DNA reassociation;Sequence homology
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	14 Mar 2008
Last Modified:	19 Sep 2010 04:43
URI:	http://eprints.iisc.ac.in/id/eprint/13394

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