Ali, Mir Ahmed and Jacob, Mathai T (1985) Characterization of Adenosine-Bovine Serum Albumin Immunogen and its Antibodies. In: Immunological Investigations, 14 (1). pp. 85-94.
Full text not available from this repository. (Request a copy)Abstract
Antibodies to adervosine were elicited in rabbits using a conjugate of adenosine (periodate oxidized) and bovine serum albumin as immunogen. The conjugate was found to be of a higher molecular size than the original protein, apparently due to the cross-linking of albumin molecules by the dialdehydes generated during the periodate oxidation of adenosine. It produced high titres of antibodies, which were 400-fold more reactive to adenosine-trialcohol than to adenosine, when measured by a nitrocellulose filter assay. In addition, a specific elution procedure for the isolation of hapten-specific antibodies is described.
| Item Type: | Journal Article |
|---|---|
| Publication: | Immunological Investigations |
| Publisher: | Taylor and Francis |
| Additional Information: | Copyright of this article belongs to Taylor and Francis |
| Keywords: | Immunology |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 11 Mar 2008 |
| Last Modified: | 27 Aug 2008 13:15 |
| URI: | http://eprints.iisc.ac.in/id/eprint/13378 |
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