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Characterization of Adenosine-Bovine Serum Albumin Immunogen and its Antibodies

Ali, Mir Ahmed and Jacob, Mathai T (1985) Characterization of Adenosine-Bovine Serum Albumin Immunogen and its Antibodies. In: Immunological Investigations, 14 (1). pp. 85-94.

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Antibodies to adervosine were elicited in rabbits using a conjugate of adenosine (periodate oxidized) and bovine serum albumin as immunogen. The conjugate was found to be of a higher molecular size than the original protein, apparently due to the cross-linking of albumin molecules by the dialdehydes generated during the periodate oxidation of adenosine. It produced high titres of antibodies, which were 400-fold more reactive to adenosine-trialcohol than to adenosine, when measured by a nitrocellulose filter assay. In addition, a specific elution procedure for the isolation of hapten-specific antibodies is described.

Item Type: Journal Article
Publication: Immunological Investigations
Publisher: Taylor and Francis
Additional Information: Copyright of this article belongs to Taylor and Francis
Keywords: Immunology
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 11 Mar 2008
Last Modified: 27 Aug 2008 13:15
URI: http://eprints.iisc.ac.in/id/eprint/13378

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