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5S-rRNA genes in rice embryos

Hariharan, N and Reddy, PS and Padayatty, JD (1987) 5S-rRNA genes in rice embryos. In: Plant Molecular Biology, 9 (5). pp. 443-451.

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The 5S-rRNA from the ungerminated and 48-h-germinated rice embryos differs from that of wheat, rye and maize by two nucleotides. The 48-h-germinated embryos contain another species of 5S-rRNA which differs by 3 nucleotides from the ungerminated embryos, thereby demonstrating the expression of two 5S-rRNA genes during germination. The 5S-rRNA genes are present in tandem repeats of a 0.3-kb sequence with some length heterogeneity in the rice genome. The 5S-rRNA gene that was sequenced is identical to that of wheat and maize, except for two nucleotides, C and T, which are interchanged at positions 107 and 117. The insert of the continuous 5S-rRNA gene in pBR322 was transcribed in vitro much more efficiently than was the discontinuous gene. There was no homology between the 184-bp spacer sequence of 5S-rRNA genes in rice and other systems except for the presence of an oligo(T) transcription terminator sequence.

Item Type: Journal Article
Publication: Plant Molecular Biology
Publisher: Springer Netherlands
Additional Information: Copyright of this article belongs to Springer Netherlands.
Keywords: gene;rice embryos;5S-rRNA;transcription
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 11 Mar 2008
Last Modified: 19 Sep 2010 04:43
URI: http://eprints.iisc.ac.in/id/eprint/13275

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