Analysis of degradation of bacterial cell division protein FtsZ by the ATP-dependent zinc-metalloprotease FtsH in vitro

Srinivasan, Ramanujam and Haryadi, Rajeswari and Parthasarathi, Ajitkumar (2008) Analysis of degradation of bacterial cell division protein FtsZ by the ATP-dependent zinc-metalloprotease FtsH in vitro. In: Microbiological Research, 163 (1). pp. 21-30.

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Abstract

The identity of protease(s), which would degrade bacterial cell division protein FtsZ in vivo, remains unknown. However, we had earlier demonstrated that Escherichia coli metalloprotease FtsH degrades E. coli cell division protein FtsZ in an ATP- and $Zn^2^+$-dependent manner in vitro. In this study, we examined FtsH protease-mediated degradation of FtsZ in vitro in detail using seven different deletion mutants of FtsZ as the substrates, which lack different extents of specific regions at the N- or C-terminus. FtsH protease assay in vitro on these mutants revealed that FtsH could degrade all the seven deletion mutants irrespective of the deletions or the extent of deletions at the N- or C-terminus. These observations indicated that neither the N-terminus nor the C-terminus was required for the degradation of FtsZ, like already known in the case of the FtsH substrate $\sigma^{32}$ protein. The recombinant clones expressing full-length FtsZ protein and FtsZ deletion mutant proteins would be useful in investigating the possibility of FtsZ as a potential in vivo substrate for FtsH in ftsH-null cells carrying ftsH suppressor function and ectopically expressed FtsH protease.

Item Type:	Journal Article
Publication:	Microbiological Research
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier
Keywords:	FtsH protease; FtsZ protein; AAA family; Bacterial cell division
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	29 Feb 2008
Last Modified:	19 Sep 2010 04:43
URI:	http://eprints.iisc.ac.in/id/eprint/13152

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