$Ca^{2+}$ ionophores inhibit superoxide generation by chemotactic peptide in rabbit neutrophils and the correlation with intracellular calcium

Jacob, Jose (1988) $Ca^{2+}$ ionophores inhibit superoxide generation by chemotactic peptide in rabbit neutrophils and the correlation with intracellular calcium. In: Molecular and Cellular Biochemistry, 84 (1). pp. 97-103.

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Abstract

Effects of $Ca^{2+}$ ionophores, A23187 and lasalocid, on superoxide anion generation by chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine methyl ester, in rabbit peritoneal exudate neutrophils were studied. The ionophores by themselves did not activate superoxide anion generation in these neutrophils. When preincubated with the cells for 2 min, both the ionophores inhibited superoxide generation induced by chemotactic peptide. The inhibition was present even in the absence of extracellular $Ca^{2+}$ and the inhibition was better then. Lasalocid produces a dose-dependent chlortetracycline fluorescence decrease response in neutrophils loaded with chlortetracycline. This response is independent of extracellular $Ca^{2+}$ concentration and is related to release of $Ca^{2+}$ from intracellular storage sites. The dose-range at which lasalocid gives this response is same as the dose-range at which it causes inhibition of superoxide response. It may be concluded that the inhibition of superoxide generation by these ionophores is correlated to intracellular $Ca^{2+}$ modulation.

Item Type:	Journal Article
Publication:	Molecular and Cellular Biochemistry
Publisher:	Springer Netherlands
Additional Information:	Copyright of this article belongs to Springer Netherlands.
Keywords:	neutrophils;A23187;lasalocid;calcium;superoxide;chemotactic peptide
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	04 Mar 2008
Last Modified:	19 Sep 2010 04:42
URI:	http://eprints.iisc.ac.in/id/eprint/13149

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