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Effect of light on nucleotide modifications in the transfer RNA of cucumber cotyledons

Raju, Putta M and Jayabaskaran, C (1988) Effect of light on nucleotide modifications in the transfer RNA of cucumber cotyledons. In: Journal of Biosciences, 13 (4). pp. 367-378.

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The effect of light on nucleotide modifications in the tRNA of cucumber (Cucumis sativus L. var. Guntur) cotyledons was studied by chromatographic, electrophoretic and immunological methods. The tRNA from light-grown tissue showed the absence of 2-methylguanosine and a decrease in the relative proportions of ribothymidine and cytokinin active ribonucleosides when compared to those produced from dark-grown tissue. On the other hand, a significant amount of one species of 2'-O-methyldinucleotide was observed in the tRNA of light-grown tissue which was not detected in the dark-grown tissue. Also, tRNA from light-grown tissue had higher levels of another species of 2'-O-methyldinucleotide. The results showed no difference in the amounts of other modified nucleosides in tRNA between tissues grown under the two conditions. 2'-O-Methyl-l-methyladenosine, a nucleotide modified both in the base and the ribose, apparently specific to plant tRNAs, has been found to be present in the RNA of both light- and dark-grown tissues. These results on the variation in modified nucleotides suggest that light has some role in nucleotide modification and, consequently, in cellular functions.

Item Type: Journal Article
Publication: Journal of Biosciences
Publisher: Springer India
Additional Information: Copyright of this article belongs to Springer.
Keywords: tRNA;light;modified nucleotides;Cucumis sativus.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 22 Apr 2008
Last Modified: 27 Aug 2008 13:11
URI: http://eprints.iisc.ac.in/id/eprint/13049

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