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Biochemical and histological validation of a model to study follicular atresia in rats

Dhanasekaran, N and Moudgal, NR (1989) Biochemical and histological validation of a model to study follicular atresia in rats. In: Endocrinologia Experimentalis, 23 (3). pp. 155-166.

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A model system to study the biochemical mechanism of follicular atresia in rats [Dhanasekaran et al. 1983] was characterized using histological and biochemical correlates. PMSG and PMSG antiserum (a/s) was used to induce the follicular growth and atresia of preovulatory follicles. Ovarian histology during these PMSG and PMSG a/s - treatment periods was recorded under a light microscope. An analysis of lysosomal enzyme cathepsin-D activity of granulosa cells (GC) from similarly treated ovaries showed that there was a reduction in cathepsin-D activity during the histologically observable follicular growth; and there was an increase in cathepsin-D activity during atresia. The increase in cathepsin-D activity also showed an inverse correlation with the general anabolic activity of the GC as demonstrated here, by a reduction in 3H-leucine incorporation activity. An analysis of other gonadotropin-responsive cells for the presence of such hormone sensitive lysosomal machinery, only corpora lutea (CL) and GC showed in the cathepsin-D activity upon treatment with 15 IU of PMSG. The results suggests the existence of a common gonadotropin regulated lysosomal machinery in cells endowed with a degenerative pathway of "programmed cell death". More importantly the results establish the validity of using lysosomal enzyme cathepsin-D as a biochemical marker, for hitherto morphologically and endocrinologically studied cellular degenerative process of follicular atresia.

Item Type: Journal Article
Publication: Endocrinologia Experimentalis
Publisher: Galenicom
Additional Information: Copyright of this article belongs to Endocrinologia experimentalis and online publisher Galenicom
Keywords: Animals, Cathepsin D, Disease Models, Animal, Female, Follicular Atresia, Follicular Phase, Gonadotropins, Equine, Granulosa Cells, Lysosomes, Ovary, Rats, Rats, Inbred Strains
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 26 Feb 2008
Last Modified: 27 Aug 2008 13:09
URI: http://eprints.iisc.ac.in/id/eprint/12842

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