Increase in hepatic microsomal lipid peroxidation mediated by $\alpha$-adrenergic system under cold stress and noradrenaline treatment

Sekhar, Seshadri B and Kurup, Ramakrishna CK and Ramasarma, T (1990) Increase in hepatic microsomal lipid peroxidation mediated by $\alpha$-adrenergic system under cold stress and noradrenaline treatment. In: Molecular and Cellular Biochemistry, 94 (1). pp. 61-70.

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Abstract

Lipid peroxidation measured both by the formation of malondialdehyde and by oxygen uptake in presence of NADPH, $Fe^{2-}$ and ADP in hepatic microsomes increased on cold exposure and decreased on heat exposure of rats. Malondialdehyde content of isolated microsomes also showed similar changes. Treatment of animals with noradrenaline or a \alpha-adrenergic agonist, phenylephrine, increased lipid peroxidation which was prevented by simultaneous treatment with cycloheximide, a protein synthesis inhibitor. Cold- and noradrenaline-induced increases were not found in animals pretreated with $\alpha$-adrenergic receptor antagonist, phenoxybenzamine, but not with propranolol, a $\beta$-adrenergic blocking agent. The concentration of the microsomal cytochromes P-450 and $b_5$ remained unaffected under these conditions but the activity of NADPH-cytochrome c reductase showed parallel changes. These observations suggest a role for lipid peroxidation in cellular thermogenesis in liver tissue.

Item Type:	Journal Article
Publication:	Molecular and Cellular Biochemistry
Publisher:	Springer Netherlands
Additional Information:	Copyright of this article belongs to Springer Netherlands.
Keywords:	lipid peroxidation;a-adrenergic receptors;non-shivering thermogenesis;noradrenaline;phenoxybenzamine;cold and heat exposure
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	03 Jan 2008
Last Modified:	19 Sep 2010 04:42
URI:	http://eprints.iisc.ac.in/id/eprint/12832

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