Immunochemical detection of Z-DNA in rat pachytene spermatocytes

Karuturi, Satyanarayana and Rao, Satyanarayana MR (1989) Immunochemical detection of Z-DNA in rat pachytene spermatocytes. In: Experimental Cell Research, 185 (2). pp. 319-326.

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Abstract

Rat testicular nuclei have been probed for the presence of Z-DNA conformation by employing indirect immunofluorescence technique using anti-Z-DNA antibodies. Pachytene nuclei, in which meiotic recombination takes place, showed brighter fluorescence than the premeiotic and postmeiotic spermatogenic nuclei. Moreover, utilizing a novel chromatin immunoblotting tecnique, Z-DNA conformation was found to be enriched in the poly(ADP-ribosyl)ated chromatin domains of the pachytene nucleus

Item Type:	Journal Article
Publication:	Experimental Cell Research
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	12 Feb 2008
Last Modified:	19 Sep 2010 04:41
URI:	http://eprints.iisc.ac.in/id/eprint/12748

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