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Regulation of protein synthesis in the first trimester human placenta by 17 \beta -oestradiol and progesterone

Sharma, Chidananda S and Kumari, Usha and Dighe, Rajan R and Rao, Jagannadha A (1990) Regulation of protein synthesis in the first trimester human placenta by 17 \beta -oestradiol and progesterone. In: Placenta, 11 (1). pp. 63-74.

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Addition of 17$\beta$-oestradiol or progesterone to first trimester human placental explants in vitro resulted in the stimulation of protein synthesis, as seen by autofluorographic analysis of placental tissue and medium proteins. An increase in the incorporation of $[^{35}S]$methionine into trichloroacetic acid-precipitable proteins was seen, following the addition of 17$\beta$-oestradiol. Use of aromatase inhibitor to block the synthesis of 17$\beta$-oestradiol inhibited the protein synthesis and while addition of cyclohexamide blocked both basal- and 17$\beta$-oestradiol-induced protein synthesis, actinomycin-D blocked only 17$\beta$-oestradiol induced protein synthesis. Double labelling of placental proteins in the presence and absence of 17$\beta$-oestradiol also indicated that there is a significant stimulation of protein synthesis by 17$\beta$-oestradiol. Based on these results it is suggested that oestradiol has a role in regulation of placental protein synthesis.

Item Type: Journal Article
Publication: Placenta
Publisher: Elsevier Ltd.
Additional Information: Copyright of this article belongs to Elsevier Ltd.
Keywords: protein synthesis;placenta;17 beta-oestradiol;progesterone
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 05 Feb 2008
Last Modified: 27 Aug 2008 13:06
URI: http://eprints.iisc.ac.in/id/eprint/12642

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